The specific genes involving castration were enriched in many pathways, including Steroid hormone biosynthesis pathway.
Both surgical and immunized castration resulted in adrenal hyperplasia, and thickness of adrenal cortex elevated. Serum testosterone concentration was significantly reduced in PBK-asd and SC group, demonstrating the effectiveness of castration. At week 14 after immunization, adrenal glands were collected for transcriptome sequencing and qPCR. Subsequently, serum was collected every two weeks after the initial immunization for hormone assays. Fifteen goats were randomly divided into three groups: pVAX-asd injection (control) group, pVAX- B2L-(G 4S) 3-kisspeptin-54-asd immunization (PBK-asd) group, and surgical castration (SC) group. This study was designed to compare the transcript profiles between adrenals from noncastrated, orchiectomized and immunocastrated Yiling goats. The content of androgen from adrenal is elevated under castration, and the mechanisms of compensatory secretion of adrenal androgen remain unknown.
